NEB, M0313S, hAAG
Catalog Number: M0313S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to create an AP site from 1 pmol of a 34-mer oligonucleotide duplex containing a single deoxyinosine site in a total reaction volume of 10 μl in 1 hour at 37°C. <b>Reaction Conditions</b> 1X ThermoPol® Reaction Buffer Incubate at 37°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.5% Tween® 20 0.5% IGEPAL® CA-630 pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Molecular Weight</b> Theoretical | 25752 daltons <b>Unit Assay Conditions</b> 1X ThermoPol Buffer containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 μl. |
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