NEB, M0315S, Terminal Transferase
Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
Catalog Number: M0315S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmol dTTP into acid-insoluble material in a total reaction volume of 50ul in 1 hour at 37°C using d(A) 18 as primer. <b>Reaction Conditions</b> 1X Terminal Transferase Reaction Buffer Pack Supplement with 0.25 mM CoCl2 Incubate at 37°C 1X Terminal Transferase Reaction Buffer Pack 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate (pH 7.9 @ 25°C) <b>Storage Buffer</b> 50 mM KPO4 100 mM NaCl 1.43 mM β-ME 50% Glycerol 0.1% Triton® X-100 pH 7.3 @ 25°C <b>Heat Inactivation</b> 75°C for 20 minutes <b>Molecular Weight</b> Theoretical | 58000 daltons <b>5' - 3' Exonuclease</b> No <b>3' - 5' Exonuclease</b> No <b>Strand Displacement</b> No <b>Unit Assay Conditions</b> 1X Terminal Transferase Reaction Buffer, 0.72 μM d(A) 18 , 0.2 mM dTTP, and 1 μCi [ 3 H]- dTTP in a 50 μl total reaction volume. |
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