NEB, M0317S, T3 DNA Ligase
T3 DNA Ligase will ligate these substrates:
Catalog Number: M0317S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to give 50% ligation of 100 ng HindIII fragments of λ DNA in a total reaction volume of 20 μl in 1 minute at 25°C in 1X StickTogether DNA Ligase Buffer. Reaction Conditions 1X StickTogether™ DNA Ligase Buffer Incubate at 25°C 1X StickTogether™ DNA Ligase Buffer 66 mM Tris-HCl 10 mM MgCl2 1 mM ATP 1 mM DTT 7.5% Polyethylene glycol (PEG 6000) (pH 7.6 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 50% Glycerol 0.1 mM EDTA pH 7.4 @ 25°C Heat Inactivation No", "value": ""}]
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