NEB, M0327S, Sulfolobus DNA Polymerase IV
Catalog Number: M0327S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 55°C. <b>Reaction Conditions</b> 1X ThermoPol® Reaction Buffer Incubate at 55°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> No <b>Molecular Weight</b> Theoretical | 40000 daltons <b>5' - 3' Exonuclease</b> No <b>3' - 5' Exonuclease</b> No <b>Strand Displacement</b> No <b>Unit Assay Conditions</b> 1X ThermoPol Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13mp18. <b>Activity at Temperature</b> @30°C: 5% @37°C: 20% @45°C: 40% @65°C: 90% @72°C: 70% |
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