NEB, M0328S, Bst DNA Polymerase, Full Length
Optimal performance in a number of applications
Catalog Number: M0328S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. Reaction Conditions 1X ThermoPol® Reaction Buffer Incubate at 65°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% Triton® X-100 pH 7.1 @ 25°C Heat Inactivation 80°C for 20 minutes 5' - 3' Exonuclease Yes 3' - 5' Exonuclease No Strand Displacement No Unit Assay Conditions 50 mM KCl, 20 mM Tris-HCl (pH 8.8), 10 mM MgCl 2 , 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24-mer, 200 µM dATP, 200 µM dCTP, 200 µM dGTP, 100 µM dTTP including [ 3 H]-dTTP and 100 µg/ml BSA.", "value": ""}]
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