NEB, M0330S, Bsu DNA Polymerase, Large Fragment
Optimal performance in a number of applications
Catalog Number: M0330S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. Reaction Conditions 1X NEBuffer™ 2 Incubate at 37°C 1X NEBuffer™ 2 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) Heat Inactivation 75°C for 20 minutes 5' - 3' Exonuclease No 3' - 5' Exonuclease No Strand Displacement + Unit Assay Conditions 1X NEBuffer 2, 33 µM dNTPs including [ 3 H]-dTTP and 70 µg/ml denatured herring sperm DNA.", "value": ""}]
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