NEB, M0331S, 5´ Deadenylase
5´ Deadenylase is capable of deadenylation from 5´-end of DNA and RNA, leaving the phosphate at 5´-end. It also cleaves AppppA into ATP and AMP.
Catalog Number: M0331S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount enzyme required to remove 10 pmoles of AMP from a 5'-adenylated DNA oligo in 10 minutes at 30°C. Reaction Conditions 1X NEBuffer™ 1 Incubate at 30°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C) Storage Buffer 10 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% (w/v) Triton® X-100 pH 7.5 @ 25°C Heat Inactivation 70°C for 20 minutes", "value": ""}]
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