NEB, M0336S, hSMUG1
Catalog Number: M0336S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to excizse 1 pmol of deoxyuracil from a 34 mer oligonucleotide duplex containing a single dU site in a total reaction volume of 10 μl in 1 hour at 37°C. <b>Reaction Conditions</b> 1X NEBuffer™ 1 Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Molecular Weight</b> Theoretical | 29861 daltons <b>Unit Assay Conditions</b> 1X NEBuffer 1 supplemented with 100 µg/ml Recombinant Albumin containing 5 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 μl. After release of dU, the oligonucleotide is cleaved by treating the resulting AP site with 100 mM NaOH for 10 minutes at 80°C. |
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