NEB, M0337S, Poly(U) Polymerase
Poly(U) Polymerase catalyzes the template independent addition of UMP from UTP or AMP from ATP to the 3´ end of RNA.
Catalog Number: M0337S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that incorporates 1 nmol of UMP into RNA in a 50 μl volume in 10 minutes at 37°C. Reaction Conditions 1X NEBuffer™ 2 Supplement with 0.5 mM UTP Incubate at 37°C 1X NEBuffer™ 2 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 65°C for 20 minutes Unit Assay Conditions 1X NEBuffer 2, 0.5 mM 3 H UTP and 5 µg yeast RNA are combined in a 50 μl reaction incubated at 37°C for 10 minutes.", "value": ""}]
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