NEB, M0345S, Exonuclease V (RecBCD)

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in 30 minutes at 37°C in a total reaction volume of 50 μl. Reaction Conditions 1X NEBuffer™ 4 Supplement with 1 mM ATP Incubate at 37°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 50 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% Triton® X-100 pH 7.5 @ 25°C Heat Inactivation 70°C for 30 minutes Unit Assay Conditions 1X NEBuffer 4, 1 mM ATP with 0.15 mM sonicated duplex [ 3 H]-DNA.", "value": ""}]