[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C. Reaction Conditions 1X Capping Buffer Supplement with 0.2 mM S-adenosylmethionine (SAM) Incubate at 37°C 1X Capping Buffer 50 mM Tris-HCl 5 mM KCl 1 mM MgCl2 1 mM DTT (pH 8 @ 25°C) Storage Buffer 20 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% (w/v) Triton® X-100 pH 8 @ 25°C", "value": ""}]
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