NEB, M0368L, ProtoScript® II Reverse Transcriptase
Catalog Number: M0368L
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Category
Pack Size0368 L
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA)•oligo(dT) 18 as template. Reaction Conditions 1X ProtoScript® II Reverse Transcriptase Reaction Buffer Incubate at 42°C 1X ProtoScript® II Reverse Transcriptase Reaction Buffer 50 mM Tris-HCl 75 mM KCl 3 mM MgCl2 (pH 8.3 @ 25°C) Storage Buffer 20 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.01% IGEPAL® CA-630 pH 7.5 @ 25°C Heat Inactivation 65°C for 20 minutes Unit Assay Conditions 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 6 mM MgCl 2 , 10 mM dithiothreitol, 0.01% IGEPAL CA-630, 0.5 mM dTTP, 0.4 mM poly(rA)•oligo(dT) 18 .", "value": ""}]
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