NEB, M0371S, Shrimp Alkaline Phosphatase (rSAP)
Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source.
Catalog Number: M0371S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of p -Nitrophenyl Phosphate, PNPP ( NEB #P0757 ) in a total reaction volume of 1 ml in 1 minute at 37°C. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Storage Buffer</b> 25 mM Tris-HCl 1 mM MgCl2 50% Glycerol pH 7.5 @ 25°C <b>Heat Inactivation</b> 65°C for 5 minutes <b>Molecular Weight</b> Theoretical | 54 kDa <b>Unit Assay Conditions</b> 1 M diethanolamine-HCl (pH 9.8), 0.5 mM MgCl 2 , 50 mM p -Nitrophenyl Phosphate. These conditions are only used for quantitating enzyme activity. |
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