[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to release 60 pmol per minute of a fluorescently labeled 47 mer single-stranded DNA oligonucleotide containing a single uracil base in 30 minutes at 30°C in a total reaction volume of 50 μl in 1X Thermopol II Buffer. Reaction Conditions 1X Standard Taq Reaction Buffer Pack Incubate at 37°C 1X Standard Taq Reaction Buffer Pack 10 mM Tris-HCl 50 mM KCl 1.5 mM MgCl2 (pH 8.3 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM NaCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 50°C for 5 minutes", "value": ""}]
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