NEB, M0374L, Bst 3.0® DNA Polymerase
Need assistance designing LAMP primers? Use the
Catalog Number: M0374L
Send an RFQ.
Category
Pack Size0374 L
Loading…
[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. Reaction Conditions 1X Isothermal Amplification Buffer II Pack Incubate at 65°C 1X Isothermal Amplification Buffer II Pack 20 mM Tris-HCl 10 mM (NH4)2SO4 150 mM KCl 2 mM MgSO4 0.1% Tween® 20 (pH 8.8 @ 25°C) Activity in NEBuffers Storage Buffer 100 mM KCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 0.1% Triton® X-100 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 80°C for 5 minutes Unit Assay Conditions 50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl 2 , 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [ 3 H]-dTTP and 100 μg/ml BSA.", "value": ""}]
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
