NEB, M0374M, Bst 3.0® DNA Polymerase
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Catalog Number: M0374M
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. Reaction Conditions 1X Isothermal Amplification Buffer II Pack Incubate at 65°C 1X Isothermal Amplification Buffer II Pack 20 mM Tris-HCl 10 mM (NH4)2SO4 150 mM KCl 2 mM MgSO4 0.1% Tween® 20 (pH 8.8 @ 25°C) Activity in NEBuffers Storage Buffer 100 mM KCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 0.1% Triton® X-100 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 80°C for 5 minutes Unit Assay Conditions 50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl 2 , 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [ 3 H]-dTTP and 100 μg/ml BSA.", "value": ""}]
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