NEB, M0375L, SplintR® Ligase
SplintR
Catalog Number: M0375L
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Category
Pack Size25 units / pack
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| <b>Materials Required but not Supplied</b> Nuclease-free Water (NEB #B1500) <b>Unit Definition</b> One unit is defined as the amount of enzyme needed to ligate (to 50% completion) 2 picomoles of a tripartite FAM-labeled DNA | RNA hybrid substrate in a 20 μl reaction at 25°C in 15 minutes in 1X SplintR Ligase Reaction Buffer. <b>Reaction Conditions</b> 1X SplintR Ligase Reaction Buffer Incubate at 25°C 1X SplintR Ligase Reaction Buffer 50 mM Tris-HCl 10 mM MgCl2 1 mM ATP 10 mM DTT (pH 7.5 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 300 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes |
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