NEB, M0378S, T3 RNA Polymerase
Bacteriophage T3 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T3 phage promoter.
Catalog Number: M0378S
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Category
Pack Size1 unit / pack
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[{"group": "General", "name": "Materials Required but not Supplied RNase Inhibitor, Murine (NEB #M0314) (optional) Ribonucleotide Solution Mix (NEB #N0466) Fresh DTT (optional) Nuclease-free Water (NEB #B1500) Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37°C. Reaction Condition 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and DNA template containing the T3 RNA Polymerase promoter. Incubate at 37°C. Reaction Conditions 1X RNAPol Reaction Buffer 1X RNAPol Reaction Buffer 40 mM Tris-HCl 6 mM MgCl2 1 mM DTT 2 mM spermidine (pH 7.9 @ 25°C) Storage Buffer 100 mM NaCl 50 mM Tris-HCl 1 mM EDTA 20 mM 2-mercaptoethanol 0.1% Triton® X-100 50% (w/v) Glycerol pH 7.9 @ 25°C Unit Assay Conditions 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and 2 μg T3 DNA in 50 μl.", "value": ""}]
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