NEB, M0379L, Exonuclease VII
Catalog Number: M0379L
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Category
Pack Size0379 L
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will catalyze the release of 1 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C. Reaction Conditions 1X Exonuclease VII Reaction Buffer Incubate at 37°C 1X Exonuclease VII Reaction Buffer 50 mM Tris-HCl 50 mM Sodium Phosphate 8 mM EDTA 10 mM 2-mercaptoethanol (pH 8 @ 25°C) Storage Buffer 50% Glycerol 50 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 0.1 M NaCl 0.1% Triton® X-100 pH 7.5 @ 25°C Heat Inactivation 95°C for 10 minutes Unit Assay Conditions 1X Exonuclease VII Reaction Buffer containing 0.15 mM sonicated single-stranded [ 3 H] E. coli DNA.", "value": ""}]
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