NEB, M0380L, WarmStart® RTx Reverse Transcriptase

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 20 minutes at 50°C using poly(rA)•oligo(dT)18 as template. Reaction Conditions 1X Isothermal Amplification Buffer Pack 1X Isothermal Amplification Buffer Pack 20 mM Tris-HCl 10 mM (NH4)2SO4 50 mM KCl 2 mM MgSO4 0.1% Tween® 20 (pH 8.8 @ 25°C) Storage Buffer 100 mM KCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 80°C for 10 minutes Unit Assay Conditions 20 mM Tris-HCl (pH 8.8), 10 mM (NH 4 ) 2 SO 4 , 50 mM KCl, 2 mM MgSO 4 , 0.1% Tween-20, 0.5 mM dTTP, 0.4 mM poly(rA)•oligo(dT)18.", "value": ""}]