NEB, M0386S, Cas9 Nuclease, S. pyogenes
Now available: EnGen Spy Cas9 HF1
Catalog Number: M0386S
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[{"group": "General", "name": "Materials Required but not Supplied User-supplied sgRNA EnGen® sgRNA Synthesis Kit, S. Pyogenes (NEB #E3322) HiScribe® Quick T7 High Yield RNA Synthesis Kit (NEB #E2050) Outside vendor Nuclease-free Water (NEB #B1500) Proteinase K, Molecular Biology Grade (NEB #P8107). Reaction Conditions 1X NEBuffer™ r3.1 Incubate at 37°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 300 mM NaCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 65°C for 5 minutes", "value": ""}]
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