NEB, M0398L, Apyrase
Apyrase (recombinant,
Catalog Number: M0398L
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Category
Pack Size000 units / pack
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that catalyses the release of 1 μmol of inorganic phosphate from ATP (1 mM, NEB #P0756 ) in 1X Apyrase Reaction Buffer in 1 minute at 30°C in a total reaction of 50 μl. <b>Reaction Conditions</b> 1X Apyrase Reaction Buffer Incubate at 30°C 1X Apyrase Reaction Buffer 20 mM MES 50 mM NaCl 5 mM CaCl2 1 mM DTT 0.05% Tween® 20 (pH 6.5 @ 25°C) <b>Storage Buffer</b> 20 mM MES 50 mM NaCl 0.1 mM CaCl2 1 mM DTT 0.1% Tween® 20 50% Glycerol pH 6.5 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Molecular Weight</b> Apparent | 47 kDa <b>Specific Activity</b> 3,000 units/mg |
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