NEB, M0402L, Bst 2.0 WarmStart® DNA Polymerase (Glycerol-free)
Catalog Number: M0402L
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Category
Pack Size0402 L
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 25 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 0.1% Triton® X-100 pH 7.1 @ 25°C Heat Inactivation 80°C for 20 minutes Unit Assay Conditions 50 mM KCl, 20 mM Tris- HCl (pH 8.8), 10 mM MgCl 2 , 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 μM dATP, 200 μM dCTP, 200 μM dGTP, 100 μM dTTP including [ 3 H]-dTTP and 100 μg/ml rAlbumin.", "value": ""}]
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