NEB, M0470T, Hi-T7® RNA Polymerase (High Concentration)
Hi-T7 RNA Polymerase is designed for
Catalog Number: M0470T
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 1 hour at 50°C Reaction Conditions 1X Hi-T7® RNA Polymerase Reaction Buffer Incubate at 50°C 1X Hi-T7® RNA Polymerase Reaction Buffer 40 mM Tris-HCl 4 mM MgCl2 50 mM NaCl 2 mM spermidine 1 mM DTT (pH 7.5 @ 25°C) Storage Buffer 50 mM Tris-HCl 100 mM NaCl 1 mM EDTA 1 mM DTT 50% Glycerol 0.1% (w/v) Triton® X-100 pH 7.9 @ 25°C Unit Assay Conditions 1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and 1 µg T7 phage DNA in 50 μl reaction volume.", "value": ""}]
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