NEB, M0480L, OneTaq® DNA Polymerase
The ONE polymerase for your endpoint PCR needs. One
Catalog Number: M0480L
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Category
Pack Size1.25 units / pack
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[{"group": "General", "name": "Materials Required but not Supplied Deoxynucleotide (dNTP) Solution Mix (NEB #N0447) Nuclease-free Water (NEB #B1500) Unit Definition One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. Reaction Conditions 1X OneTaq® Standard Reaction Buffer Pack 1X OneTaq® Standard Reaction Buffer Pack 20 mM Tris-HCl 22 mM NH4Cl 22 mM KCl 1.8 mM MgCl2 0.06% IGEPAL® CA-630 0.05% Tween® 20 (pH 8.9 @ 25°C) Usage Concentration 1.25 units/50μl reaction Storage Buffer 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.5% Tween® 20 0.5% IGEPAL® CA-630 pH 7.4 @ 25°C Heat Inactivation No 5' - 3' Exonuclease Yes 3' - 5' Exonuclease Yes Strand Displacement No Unit Assay Conditions 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA Error Rate < 140x10 -6 bases", "value": ""}]
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