NEB, M0480X, OneTaq® DNA Polymerase
The ONE polymerase for your endpoint PCR needs. One
Catalog Number: M0480X
Send an RFQ.
Category
Pack Size1.25 units / pack
Loading…
[{"group": "General", "name": "Materials Required but not Supplied Deoxynucleotide (dNTP) Solution Mix (NEB #N0447) Nuclease-free Water (NEB #B1500) Unit Definition One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. Reaction Conditions 1X OneTaq® Standard Reaction Buffer Pack 1X OneTaq® Standard Reaction Buffer Pack 20 mM Tris-HCl 22 mM NH4Cl 22 mM KCl 1.8 mM MgCl2 0.06% IGEPAL® CA-630 0.05% Tween® 20 (pH 8.9 @ 25°C) Usage Concentration 1.25 units/50μl reaction Storage Buffer 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.5% Tween® 20 0.5% IGEPAL® CA-630 pH 7.4 @ 25°C Heat Inactivation No 5' - 3' Exonuclease Yes 3' - 5' Exonuclease Yes Strand Displacement No Unit Assay Conditions 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA Error Rate < 140x10 -6 bases", "value": ""}]
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
