[{"group": "General", "name": "Materials Required but not Supplied Nuclease-free Water (NEB #B1500) 1X Master Mix Composition 20 mM Tris-HCl 22 mM KCl 22 mM NH4Cl 1.8 mM MgCl2 5% Glycerol 0.05% Tween® 20 0.06% IGEPAL® CA-630 0.2 mM dNTPs 25 units/ml OneTaq® DNA Polymerase pH 8.9 @ 25°C Heat Inactivation No Unit Assay Conditions 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.", "value": ""}]
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