NEB, M0489L, OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer<br/>
Catalog Number: M0489L
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Category
Pack Size25 units / pack
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| <b>1X Master Mix Composition</b> 80 mM Tris-SO4 1X Tartrazine 25 units/ml OneTaq Hot Start DNA Polymerase 20 mM (NH4)2SO4 0.2 mM dNTPs 2 mM MgSO4 5% Glycerol 5% DMSO 0.06% IGEPAL® CA-630 0.05% Tween® 20 1X Xylene Cyanol pH 9.2 @ 25°C <b>Heat Inactivation</b> No <b>Unit Assay Conditions</b> 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA. Unit Definition | One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. Reaction Conditions: 1X One Taq Hot Start Quick-Load Master Mix with GC Buffer, DNA template and primers in a total reaction volume of 50 μl. |
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