NEB, M0490L, EpiMark® Hot Start Taq DNA Polymerase
DNA Polymerase optimized for epigenetics analysis
Catalog Number: M0490L
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Category
Pack Size2 units / pack
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that will incorporate 15 nmol dNTP into acid insoluble material in 30 minutes at 75°C. <b>Reaction Conditions</b> 1X EpiMark® Hot Start Taq Reaction Buffer Pack 1X EpiMark® Hot Start Taq Reaction Buffer Pack 20 mM Tris-HCl 1.8 mM MgCl2 22 mM KCl 0.06% IGEPAL® CA-630 0.05% Tween® 20 22 mM NH4Cl (pH 8.9 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 0.5% Tween® 20 0.5% IGEPAL® CA-630 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> No <b>Molecular Weight</b> Theoretical | 94000 daltons <b>5' - 3' Exonuclease</b> Yes <b>3' - 5' Exonuclease</b> No <b>Unit Assay Conditions</b> 1X ThermoPol ® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA. |
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