| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 pmol of a fluorescently labeled 25 base pair RNA | DNA hybrid in a total reaction volume of 50 μl in 20 minutes at 50°C. <b>Reaction Conditions</b> 1X RNase H Reaction Buffer Incubate at ≥50°C 1X RNase H Reaction Buffer 50 mM Tris-HCl 75 mM KCl 3 mM MgCl2 10 mM DTT (pH 8.3 @ 25°C) <b>Storage Buffer</b> 50 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 0.1% Triton® X-100 50% Glycerol pH 7.5 @ 25°C <b>Heat Inactivation</b> No |
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