NEB, M0525S, Quick CIP
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Catalog Number: M0525S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of p-Nitrophenyl Phosphate, PNPP ( NEB #P0757 ) in a total reaction volume of 1 ml in 1 minute at 37°C. Reaction Conditions 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 25 mM Tris-HCl 1 mM MgCl2 0.1 mM ZnCl2 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 80°C for 2 minutes Unit Assay Conditions 1 M diethanolamine-HCl (pH 9.8), 0.5 mM MgCl 2 , 50 mM p-Nitrophenyl Phosphate. These conditions are only used for quantitating enzyme activity.", "value": ""}]
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