NEB, M0530S, Phusion™ High-Fidelity DNA Polymerase
For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific
Catalog Number: M0530S
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Category
Pack Size2 units / pack
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[{"group": "General", "name": "Materials Required but not Supplied Deoxynucleotide (dNTP) Solution Mix (NEB #N0447) Nuclease-free Water (NEB #B1500) Template DNA Associated forward and reverse primers Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C. Storage Buffer 20 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol 1X stabilizers pH 7.4 @ 25°C Heat Inactivation No 5' - 3' Exonuclease No 3' - 5' Exonuclease Yes Unit Assay Conditions 25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl 2 , 1 mM b-mercaptoethanol, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA.", "value": ""}]
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