| <b>Heat Inactivation</b> No <b>Unit Assay Conditions</b> 25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl 2 , 1 mM β-mercaptoethanol, 200 μM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA. Unit Definition | One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C. Reaction Conditions: 1X Phusion High-Fidelity PCR Master Mix with GC Buffer, DNA template, 0.5 µM primers and 3% DMSO (optional) in a total reaction volume of 50 μl. |
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