NEB, M0535L, Phusion™ Hot Start Flex DNA Polymerase
Catalog Number: M0535L
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Category
Pack Size2 units / pack
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C. Storage Buffer 20 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol 1X stabilizers pH 7.4 @ 25°C Heat Inactivation No 5' - 3' Exonuclease No Unit Assay Conditions 25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl 2 , 1 mM β-mercaptoethanol, 200 μM dNTPs including [ 3 H]- dTTP and 15 nM primed M13 DNA.", "value": ""}]
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