NEB, M0550S, E. coli RNA Polymerase, Core Enzyme
RNA Polymerase, Core Enzyme consists of 5 subunits designated α, α, β´, β, and ω.
Catalog Number: M0550S
Send an RFQ.
Category
Loading…
[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol NTP into RNA in 10 minutes at 37°C. Reaction Conditions 1X E.coli RNA Polymerase Reaction Buffer Incubate at 37°C 1X E.coli RNA Polymerase Reaction Buffer 40 mM Tris-HCl 150 mM KCl 10 mM MgCl2 1 mM DTT 0.01% Triton® X-100 (pH 7.5 @ 25°C) Storage Buffer 20 mM Tris-HCl 100 mM NaCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.5 @ 25°C Unit Assay Conditions 1X E. coli RNA Polymerase Reaction Buffer, supplemented with 0.5 mM of each NTP, sigma factor 70 and 1 μg T7 phage DNA in 50 μl.", "value": ""}]
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
