[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol NTP into RNA in 10 minutes at 37°C. Reaction Conditions 1X E.coli RNA Polymerase Reaction Buffer Incubate at 37°C 1X E.coli RNA Polymerase Reaction Buffer 40 mM Tris-HCl 150 mM KCl 10 mM MgCl2 1 mM DTT 0.01% Triton® X-100 (pH 7.5 @ 25°C) Storage Buffer 20 mM Tris-HCl 100 mM NaCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.5 @ 25°C Unit Assay Conditions 1X E. coli RNA Polymerase Reaction Buffer, supplemented with 0.5 mM of each NTP and 1 μg T7 phage DNA in 50 μl.", "value": ""}]
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