NEB, M0568L, Thermolabile Exonuclease I
Interested in a 5-minute protocol? Try out the Exo-CIP
Catalog Number: M0568L
Send an RFQ.
Category
Pack Size0568 L
Loading…
[{"group": "General", "name": "Unit Definition One unit of Thermolabile Exonuclease I is defined as the amount of enzyme that will catalyze the release of 2 nmol of acid-soluble nucleotide in a total reaction volume of 100 μl in 6 minutes at 37°C in 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl 2 and 100 μg/ml BSA with 0.17 mg/ml single-stranded [3H]- E. coli DNA. Reaction Conditions 1X NEBuffer™ r3.1 Incubate at 37°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 0.25 M NaCl 50% Glycerol 200 µg/ml BSA pH 7.4 @ 25°C Heat Inactivation 80°C for 1 minute Unit Assay Conditions 50 mM Tris-HCl (pH 7.9), 10 mM MgCl2, 100 mM NaCl, 100 μg/ml BSA and 0.17 mg/ml single-stranded [3H]- E.coli DNA.", "value": ""}]
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
