NEB, M0568S, Thermolabile Exonuclease I
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Catalog Number: M0568S
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[{"group": "General", "name": "Unit Definition One unit of Thermolabile Exonuclease I is defined as the amount of enzyme that will catalyze the release of 2 nmol of acid-soluble nucleotide in a total reaction volume of 100 μl in 6 minutes at 37°C in 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl 2 and 100 μg/ml BSA with 0.17 mg/ml single-stranded [3H]- E. coli DNA. Reaction Conditions 1X NEBuffer™ r3.1 Incubate at 37°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 0.25 M NaCl 50% Glycerol 200 µg/ml BSA pH 7.4 @ 25°C Heat Inactivation 80°C for 1 minute Unit Assay Conditions 50 mM Tris-HCl (pH 7.9), 10 mM MgCl2, 100 mM NaCl, 100 μg/ml BSA and 0.17 mg/ml single-stranded [3H]- E.coli DNA.", "value": ""}]
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