NEB, M0603S, EcoGII Methyltransferase

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to protect 100 ng FAM-labeled dsDNA in 30 minutes at 37°C in a total reaction volume of 20 μl against cleavage by MboI restriction endonuclease. Reaction Conditions 1X rCutSmart™ Buffer Supplement with 160 µM S-adenosylmethionine (SAM) Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 0.15% Triton® X-100 180 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 65°C for 10 minutes Unit Assay Conditions EcoGII Methyltransferase is incubated with 100 ng of a FAM-labeled dsDNA substrate (80 bp) in 20 μl of 1X rCutSmart Buffer supplemented with 160 µM SAM, for 30 minutes at 37°C followed by 10 minutes at 65°C. The extent of protection is determined by addition of 5 units of MboI restriction endonuclease (incubation at 37°C for 30 minutes, 65°C for 20 minutes) followed by capillary electrophoresis analysis to resolve the reaction products, assign the cleaved and uncleaved species and quantitate their abundance.", "value": ""}]