[{"group": "General", "name": "Unit Definition 1 µM of NudC hydrolyzes 200 µM or more NAD + into NMN + and AMP in 1X NEBuffer 3.1 and 5 mM DTT at 37°C for 30 min. Reaction Conditions 1X NEBuffer™ r3.1 Supplement with 5 mM DTT Incubate at 37°C NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 300 mM NaCl 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.9 @ 25°C Heat Inactivation 65°C for 10 minutes", "value": ""}]
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