NEB, M0608S, mRNA Decapping Enzyme
mRNA Decapping Enzyme decaps m
Catalog Number: M0608S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of mRNA Decapping Enzyme required to convert 50% of a 500 nM m 7 G-capped substrate to a 5´-monophosphorylated form in a total reaction volume of 20 μl in 1 hour at 37˚C. Reaction Conditions 1X mRNA Decapping Enzyme Reaction Buffer Incubate at 37°C 1X mRNA Decapping Enzyme Reaction Buffer 50 mM Tris-HCl 50 mM Ammonium Chloride 5 mM MgCl2 1 mM DTT 0.1% Poloxamer 188 (pH 7.5 @ 25°C) Storage Buffer 300 mM NaCl 10 mM Tris-HCl 0.1 mM DTT 50% Glycerol pH 7.4 @ 25°C Heat Inactivation No", "value": ""}]
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