[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to cleave 0.5 μg of pMiniT-TelN BsaI-linearized control plasmid (313 fmol of TelN recognition sites) in a total reaction volume of 50 μl in 30 minutes at 30°C in 1X ThermoPol Reaction Buffer. Reaction Conditions 1X ThermoPol® Reaction Buffer Incubate at 30°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) Heat Inactivation 75°C for 5 minutes", "value": ""}]
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