NEB, M0658S, Hi-T7® RNA Polymerase

[{"group": "General", "name": "Materials Required but not Supplied RNase Inhibitor, Murine (NEB #M0314) (optional) Ribonucleotide Solution Mix (NEB #N0466) Nuclease-free Water (NEB #B1500) Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol of ATP into acid-insoluble material in 1 hour at 50°C. Reaction Conditions 1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each of ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37-56°C for 1 hour. Optimal incubation temperature is 50-52°C. Reaction Conditions 1X Hi-T7® RNA Polymerase Reaction Buffer Supplement with 0.5 mM ATP, 0.5 mM UTP , 0.5 mM GTP and 0.5 mM CTP Incubate at 50°C 1X Hi-T7® RNA Polymerase Reaction Buffer 40 mM Tris-HCl 4 mM MgCl2 50 mM NaCl 2 mM spermidine 1 mM DTT (pH 7.5 @ 25°C) Storage Buffer 50 mM Tris-HCl 100 mM NaCl 1 mM EDTA 1 mM DTT 0.1% (w/v) Triton® X-100 50% Glycerol pH 7.9 @ 25°C Unit Assay Conditions 1X Hi-T7 RNA Polymerase Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP and 1 µg T7 phage DNA in 50 μl reaction volume.", "value": ""}]