NEB, M0659S, 5-hydroxymethyluridine DNA Kinase

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to protect 1 µg of Bacillus subtilis bacteriophage SP8 genomic DNA in 30 minutes at 37°C in a total reaction volume of 20 μl against subsequent cleavage by NcoI-HF restriction endonuclease. Reaction Conditions 1X T4 DNA Ligase Reaction Buffer Incubate at 37°C 1X T4 DNA Ligase Reaction Buffer 50 mM Tris-HCl 10 mM MgCl2 1 mM ATP 10 mM DTT (pH 7.5 @ 25°C) Storage Buffer 300 mM NaCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 80°C for 10 minutes Unit Assay Conditions 1X T4 DNA Ligase Reaction Buffer, 1 µg of Bacillus subtilis bacteriophage SP8 genomic DNA in a total reaction volume of 20 μl. The reaction mix is incubated for 30 minutes at 37°C and then incubated at 80°C for 10 minutes. Confirmation of phosphorylation is determined by protection of subsequent cleavage by NcoI-HF restriction endonuclease. The reaction mix is treated with 2 μl of 10X rCutSmart Buffer and 20 units of NcoI-HF restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on an agarose gel.", "value": ""}]