[{"group": "General", "name": "Unit Definition 1 unit of T5 Exonuclease is defined as the amount of enzyme required to cause the change of 0.00032 A260 nm/min at 37° C in rCutSmart Buffer. Reaction Conditions 1X NEBuffer™ 4 Incubate at 37°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 50 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% Triton® X-100 pH 7.5 @ 25°C Heat Inactivation No Unit Assay Conditions 1X CutSmart Buffer; 40 µg/ml Salmon Sperm DNA", "value": ""}]
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