NEB, M0678S, Mismatch Endonuclease I
Catalog Number: M0678S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to cleave 50% of 0.2 pmol of a fluorescently labeled 60mer oligonucleotide duplex containing a single T | T mismatch in 30 minutes at 37°C in a total reaction volume of 20 μl in 1X NEBuffer r2.1. <b>Reaction Conditions</b> 1X NEBuffer™ r2.1 Incubate at 37°C NEBuffer™ r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 500 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 70°C for 5 minutes |
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