NEB, M0701S, Thermostable Endonuclease Q
Catalog Number: M0701S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to cleave 1 pmol of a 60-mer oligonucleotide ssDNA containing a single deoxyxanthosine site* in a total reaction volume of 20 μl after 1 hour at 65°C. *A deoxyxanthosine site is synthetically prepared with dX at the 24th position of a 5´ FAM-labelled 60-mer ssDNA oligo. Reaction Conditions 1X NEBuffer™ r2.1 Incubate at 65°C 1X NEBuffer™ r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 500 mM NaCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.4 @ 25°C Heat Inactivation No", "value": ""}]
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