NEB, M0766L, Thymine-DNA Glycosylase (TDG)
Catalog Number: M0766L
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Pack Size0766 L
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to create an AP site from 1 pmol of a 60-mer oligonucleotide duplex containing a single T | G mispair within a CpG context* in a total reaction of 20 μl in 1 hour at 37°C. *A T:G mispair is prepared by having the T located at the 24th position of a 5′ FAM-labelled 60 mer ssDNA and a G at the 25th position of an unlabeled complement, annealed to form a duplex substrate. <b>Reaction Conditions</b> 1X TDG Reaction Buffer Incubate at 37°C 1X TDG Reaction Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 0.25 mM TCEP 10 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 500 mM NaCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 10 minutes |
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