NEB, M0766S, Thymine-DNA Glycosylase (TDG)
Catalog Number: M0766S
Send an RFQ.
Category
Loading…
| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to create an AP site from 1 pmol of a 60-mer oligonucleotide duplex containing a single T | G mispair within a CpG context* in a total reaction of 20 μl in 1 hour at 37°C. *A T:G mispair is prepared by having the T located at the 24th position of a 5′ FAM-labelled 60 mer ssDNA and a G at the 25th position of an unlabeled complement, annealed to form a duplex substrate. <b>Reaction Conditions</b> 1X TDG Reaction Buffer Incubate at 37°C 1X TDG Reaction Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 0.25 mM TCEP 10 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 500 mM NaCl 0.1 mM EDTA 1 mM DTT 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 10 minutes |
|---|
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
