NEB, M1282S, WarmStart® Afu Uracil-DNA Glycosylase (UDG)
Catalog Number: M1282S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to release 60 pmol per minute of a fluorescently labeled 47 mer single-stranded DNA oligonucleotide containing a single uracil base in 30 minutes at 65°C in a total reaction volume of 50 μl in 1X Thermopol II Buffer. <b>Reaction Conditions</b> 1X ThermoPol® II (Mg-free) Reaction Buffer Incubate at 65°C 1X ThermoPol® II (Mg-free) Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 0.1% Triton® X-100 (pH 8.8 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 0.1 mg/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> No <b>Molecular Weight</b> Theoretical | 22717 daltons |
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